Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy.

نویسندگان

  • Alberto Rodríguez-Pulido
  • Aitziber L Cortajarena
  • Joaquim Torra
  • Rubén Ruiz-González
  • Santi Nonell
  • Cristina Flors
چکیده

Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

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عنوان ژورنال:
  • Chemical communications

دوره 52 59  شماره 

صفحات  -

تاریخ انتشار 2016